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The objective of the present study was to develop a rapid, high-throughput γ-H2AX assay based on imaging flow cytometry (IFC) using the ImageStream® X Mk II (ISX) platform to evaluate DNA double strand break (DSB) repair kinetics in human peripheral blood cells after exposure to ionizing irradiation. In contrast, flow cytometry allows simple detection of gamma-H2AX in a large number of cells (31). Several reports show that the level of gamma-H2AX as detected by flow cytometry correlates well with the number of DNA strand breaks, to the level of cell death and radiosensitivity (32–34). Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. This process is believed to play a key role in the repair of DNA damage.

Gamma h2ax flow cytometry

The compounds were classified as true genotoxicants, nongenotoxicants and false genotoxicants showing positive results in in vitro genotoxicity assays, but negative results in in vivo genotoxicity assays. H2A.X Phosphorylation Assay Kit (Flow Cytometry) Overview: Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X. Lastly, flow cytometry has been used to analyze γ-H2AX; however, flow cytometry methods are not readily integrated into HTS. Although each of the aforementioned methods of evaluating γ-H2AX is effective and has provided important information, there is still a need for an analytical high throughput assay that is capable of screening radiomodifying drugs across diverse cell lines and in vivo 2011-09-23 · Here, measurements of γ-H2AX immunofluorescence by microscopy and flow cytometry were compared as rapid biodosimetric tools for whole and partial body exposures. Ex vivo uniformly X-irradiated blood lymphocytes from one donor were used to generate a universal biexponential calibration function for γ-H2AX foci/intensity yields per unit dose for time points up to 96 hours post exposure. 2010-02-04 · Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin.

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Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s). Rabbit polyclonal gamma H2A.X (phospho S139) antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Human.

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Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products.

94.88%. 99.29%. Polyclonal Antibody for studying H2AX (Ser139) phosphate in the Chromatin Immunoprecipitation; IF-Immunofluorescence; F-Flow Cytometry; E-P-ELISA- Flow cytometry detection successfully revealed a rapid and time‐dependent H2AX phosphorylation on Ser139 in MNNG‐treated cells (Figure 3A).
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Studies have suggested gamma H2AX quantification by immunoflourescence as a useful biomarker of human low level radiation exposure. In immunofluorescence method numbers of foci formed are individually counted by microscopic evaluation.

Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers. Showing 9 of 9 suppliers (63 products total) <<.
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Lastly, flow cytometry has been used to analyze γ-H2AX; however, flow cytometry methods are not readily integrated into HTS. Although each of the aforementioned methods of evaluating γ-H2AX is effective and has provided important information, there is still a need for an analytical high throughput assay that is capable of screening radiomodifying drugs across diverse cell lines and in vivo 2011-09-23 · Here, measurements of γ-H2AX immunofluorescence by microscopy and flow cytometry were compared as rapid biodosimetric tools for whole and partial body exposures. Ex vivo uniformly X-irradiated blood lymphocytes from one donor were used to generate a universal biexponential calibration function for γ-H2AX foci/intensity yields per unit dose for time points up to 96 hours post exposure.